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    OpenSpecimen Forums

    Passaging Cell Lines in OpenSpecimen

    Technical Questions
    #1

    Hi OpenSpecimen Community,

    I was curious how you or your end users record making passages of a cell line. Some researchers use a technique of passaging with fibroblasts, basically creating aliquots of an aliquot. We have an end user that can have up to 40+ passages of their primary cell lines. Is there a better way to do this in OpenSpecimen besides creating aliquots of aliquots? I’m thinking ahead that the labels for these specimens could get very long, very quickly. If we turn off label tokens, then they would have to but the unique labels in, but they are processing a lot of samples in the CP event. I’d welcome any ideas or advice for this kind of topic.
    Thanks!

    #2

    Hi Sean,
    There are multiple ways to track cell line passaging in OpenSpecimen.

    Here is a wiki link for your reference: Representing Cell Line Passaging in OpenSpecimen: Options and Considerations

    Regards,
    Rashmi

    #3

    Hi Rashmi,

    Thank you for the link. One question for follow-up, are you able to provide more concise instructions on Option 2? " Steps: Configure the Collection Protocol (CP) to allow for multiple, flat-level derivatives. Use custom label tokens (e.g., %PSPEC_LABEL%.P%PSPEC_UID%) to ensure consistent, readable labeling. This will create labels like <parent_label>.P1, <parent_label>.P2, etc."

    • Configure the CP how?

    • Does the label token to in at the SRC level/ specimen requirement label format? If it goes into the general aliquot token, not all aliquots are passages.